Statistical inference from multiple iTRAQ experiments without using common reference standards.

ABSTRACT: Isobaric tags for relative and absolute quantitation (iTRAQ) is a prominent mass spectrometry technology for protein identification and quantification that is capable of analyzing multiple samples in a single experiment. Frequently, iTRAQ experiments are carried out using an aliquot from a pool of all samples, or "masterpool", in one of the channels as a reference sample standard to estimate protein relative abundances in the biological samples and to combine abundance estimates from multiple experiments. In this manuscript, we show that using a masterpool is counterproductive. We obtain more precise estimates of protein relative abundance by using the available biological data instead of the masterpool and do not need to occupy a channel that could otherwise be used for another biological

SUBMITTER: Herbrich SM 

PROVIDER: S-EPMC4223774 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature