The disulfide isomerase ERp57 is required for fibrin deposition in vivo.

ABSTRACT:

Background

ERp57 is required for platelet function; however, whether ERp57 contributes to fibrin generation is unknown.

Methods and results

Using an inhibitory anti-ERp57 antibody (mAb1), Pf4-Cre/ERp57(fl/fl) mice, Tie2-Cre/ERp57(fl/fl) mice, and mutants of ERp57, we analyzed the function of ERp57 in laser-induced thrombosis. Fibrin deposition was decreased in Pf4-Cre/ERp57(fl/fl) mice, consistent with a role for platelet ERp57 in fibrin generation. Fibrin deposition was further decreased with infusion of mAb1 and in Tie2-Cre/ERp57(fl/fl) mice, consistent with endothelial cells also contributing to fibrin deposition. Infusion of eptibifatide inhibited platelet and fibrin deposition, confirming a role for platelets in fibrin deposition. Infusion of recombinant ERp57 corrected

SUBMITTER: Zhou J 

PROVIDER: S-EPMC4229395 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature