Multiple roles of the PGE2 -EP receptor signal in vascular permeability.

ABSTRACT:

Background and purpose

PGE2 is a major prostanoid that regulates inflammation by stimulating EP1-4 receptors. However, how PGE2 induces an initial inflammatory response to vascular hyper-permeability remains unknown. Here we investigated the role of the PGE2 -EP receptor signal in modulating vascular permeability both in vivo and in vitro.

Experimental approach

We used a modified Miles assay and intravital microscopy to examine vascular permeability in vivo. Endothelial barrier property was assessed by measuring transendothelial electrical resistance (TER) in vitro.

Key results

Local administration of PGE2 , an EP2 or EP4 receptor agonist into FVB/NJcl mouse ear skin caused vascular leakage, indicated by dye extravasation. Intravital microscopy and laser Doppler blood

SUBMITTER: Omori K 

PROVIDER: S-EPMC4294111 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature