Identification of stroke-associated-antigens via screening of recombinant proteins from the human expression cDNA library (SEREX).

ABSTRACT:

Background

Because circulating antibodies against a variety of antigens have been detected in patients with coronary heart disease, carotid atherosclerosis and those who have suffered a stroke, it is suspected that immune response may be one of the mechanisms of atherogenesis The objective of this study is to identify novel antibodies in ischemic stroke patients by screening the expressed recombinant proteins using a human cDNA library (SEREX).

Methods

To identify the candidate antigens, cDNA library was screened by SEREX using plasma from ten patients with ischemic stroke. Subsequently, via ELISA using recombinant proteins and synthetic peptides, the serum antibody levels were measured in two independent patient/healthy donor (HD) cohorts (142 and 78 in the 2nd screening an

SUBMITTER: Machida T 

PROVIDER: S-EPMC4344740 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature