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Ultrastructurally smooth thick partitioning and volume stitching for large-scale connectomics.

ABSTRACT: Focused-ion-beam scanning electron microscopy (FIB-SEM) has become an essential tool for studying neural tissue at resolutions below 10 nm × 10 nm × 10 nm, producing data sets optimized for automatic connectome tracing. We present a technical advance, ultrathick sectioning, which reliably subdivides embedded tissue samples into chunks (20 ?m thick) optimally sized and mounted for efficient, parallel FIB-SEM imaging. These chunks are imaged separately and then 'volume stitched' back together, producing a final three-dimensional data set suitable for connectome tracing.

SUBMITTER: Hayworth KJ

PROVIDER: S-EPMC4382383 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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Ultrastructurally smooth thick partitioning and volume stitching for large-scale connectomics.

Hayworth Kenneth J KJ Xu C Shan CS Lu Zhiyuan Z Knott Graham W GW Fetter Richard D RD Tapia Juan Carlos JC Lichtman Jeff W JW Hess Harald F HF

Nature methods 20150216 4

Focused-ion-beam scanning electron microscopy (FIB-SEM) has become an essential tool for studying neural tissue at resolutions below 10 nm × 10 nm × 10 nm, producing data sets optimized for automatic connectome tracing. We present a technical advance, ultrathick sectioning, which reliably subdivides embedded tissue samples into chunks (20 μm thick) optimally sized and mounted for efficient, parallel FIB-SEM imaging. These chunks are imaged separately and then 'volume stitched' back together, pro ...[more]

PMID: 25686390

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