Blockade of MMP14 activity in murine breast carcinomas: implications for macrophages, vessels, and radiotherapy.

ABSTRACT: Matrix metalloproteinase (MMP) 14 may mediate tumor progression through vascular and immune-modulatory effects. Orthotopic murine breast tumors (4T1 and E0771 with high and low MMP14 expression, respectively; n = 5-10 per group) were treated with an anti-MMP14 inhibitory antibody (DX-2400), IgG control, fractionated radiation therapy, or their combination. We assessed primary tumor growth, transforming growth factor β (TGFβ) and inducible nitric oxide synthase (iNOS) expression, macrophage phenotype, and vascular parameters. A linear mixed model with repeated observations, with Mann-Whitney or analysis of variance with Bonferroni post hoc adjustment, was used to determine statistical significance. All statistical tests were two-sided. DX-2400 inhibited tumor growth compared with IgG contro

SUBMITTER: Ager EI 

PROVIDER: S-EPMC4402365 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature