Characterization of a novel radiation-induced sarcoma cell line.

ABSTRACT:

Background

Radiation-induced sarcoma (RIS) is a potential complication of cancer treatment. No widely available cell line models exist to facilitate studies of RIS.

Methods

We derived a spontaneously immortalized primary human cell line, UACC-SARC1, from a RIS.

Results

Short tandem repeat (STR) profiling of UACC-SARC1 was virtually identical to its parental tumor. Immunohistochemistry (IHC) analysis of the tumor and immunocytochemistry (ICC) analysis of UACC-SARC1 revealed shared expression of vimentin, osteonectin, CD68, Ki67 and PTEN but tumor-restricted expression of the histiocyte markers α1-antitrypsin and α1-antichymotrypsin. Karyotyping of the tumor demonstrated aneuploidy. Comparative genomic hybridization (CGH) provided direct genetic comparison between the t

SUBMITTER: Lang J 

PROVIDER: S-EPMC4406876 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature