Alternative surfactants for improved efficiency of in situ tryptic proteolysis of fingermarks.

ABSTRACT: Despite recent improvements to in situ proteolysis strategies, a higher efficiency is still needed to increase both the number of peptides detected and the associated ion intensity, leading to a complete and reliable set of biomarkers for diagnostic or prognostic purposes. In the study presented here, an extract of a systematic study is illustrated investigating a range of surfactants assisting trypsin proteolytic activity. Method development was trialled on fingermarks; this specimen results from a transfer of sweat from an individual's fingertip to a surface upon contact. As sweat carries a plethora of biomolecules, including peptides and proteins, fingermarks are, potentially, a very valuable specimen for non-invasive prognostic or diagnostic screening. A recent study has demonstrated t

SUBMITTER: Patel E 

PROVIDER: S-EPMC4422860 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature