Genetic polymorphisms of the sortase A gene and social-behavioural factors associated with caries in children: a case-control study.

ABSTRACT:

Background

Streptococcus mutans (S. mutans) is the primary etiological agent of dental caries. Sortase is a transpeptidase that anchors several surface proteins to the S. mutans cell wall and has been shown to play a major role in cariogenicity. The purpose of this study was to explore the genetic polymorphisms of the sortase gene (srtA) and the social-behavioural factors associated with dental caries in children with S. mutans.

Methods

In this case-control study, 121 S. mutans strains were separately selected from caries-free children and high-severity caries children for sequencing of the srtA gene. Social and behavioural data were collected by self-administered questionnaires. Genomic DNA was extracted from S. mutans strains and amplified by PCR to obtain the srtA gene. T

SUBMITTER: Yu LX 

PROVIDER: S-EPMC4423529 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature