Bimodal in vivo imaging provides early assessment of stem-cell-based photoreceptor engraftment.

ABSTRACT:

Purpose

Subretinal transplantation of stem-cell-derived photoreceptor precursor cells (PPCs) is a promising and innovative approach to treating a range of blinding diseases. However, common barriers to efficient preclinical transplantation comes in the form of suboptimal graft architecture, limited graft survival, and immune-rejection, each of which cannot be assessed using conventional in vivo imaging (i.e., rodent ophthalmoscopy). With the majority of PPCs reported to die within the first few weeks after transplantation, understanding the mechanisms of graft failure, and ultimately devising preventative methods, currently relies on lengthy end point histology. To address these limitations, we hypothesized that combining two imaging modalities, optical coherence tomography (OCT) a

SUBMITTER: Laver CR 

PROVIDER: S-EPMC4429275 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature