Effect of NS398 on metastasis-associated gene expression in a human colon cancer cell line.

ABSTRACT:

Aim

To investigate the effect of NS398 on the metastasis-associated gene expression in LoVo colorectal cancer cells.

Methods

LoVo cells were treated with NS398 at the concentration of 100 micromol/L for 24 and 48 h respectively. Total RNA was extracted with TRIzol reagents and reverse transcribed with Superscript II and hybridized with cDNA microarray (containing oncogenes, tumor suppressor genes, signal transduction molecules, adhesive molecules, growth factors, and ESTs) fabricated in our laboratory. After normalization, the ratio of gene expression of NS398 treated to untreated LoVo cells was either 2-fold up or 0.5-fold down was defined as the differentially expressed genes. Semi-quantitative RT-PCR was used to validate the microarray results.

Results

Among the 44

SUBMITTER: Gao XQ 

PROVIDER: S-EPMC4434659 | biostudies-literature | 2005 Jul

REPOSITORIES: biostudies-literature