Dataset Information
De novo protein structure determination from near-atomic-resolution cryo-EM maps.
Ontology highlight
ABSTRACT:
SUBMITTER: Wang RY
PROVIDER: S-EPMC4435692 | biostudies-literature | 2015 Apr
REPOSITORIES: biostudies-literature
Publications
De novo protein structure determination from near-atomic-resolution cryo-EM maps.
Nature methods 20150223 4
We present a de novo model-building approach that combines predicted backbone conformations with side-chain fit to density to accurately assign sequence into density maps. This method yielded accurate models for six of nine experimental maps at 3.3- to 4.8-Å resolution and produced a nearly complete model for an unsolved map containing a 660-residue heterodimeric protein. This method should enable rapid and reliable protein structure determination from near-atomic-resolution cryo-electron micros ...[more]