Optimization studies for enhanced bacteriocin production by Pediococcus pentosaceus KC692718 using response surface methodology.

ABSTRACT: Bacteriocins have been produced by various Lactic acid bacteria (LAB) strains isolated from dairy and fermented vegetable sources. In the current study we have isolated a novel bacteriocin producing strain Pediococcus pentosaceus KC692718 from mixed vegetable pickles (India). A 2 step process optimization for enhancing production of bacteriocin from the isolates was carried out with One-factor-at-a-time (OFAT) and Response Surface Methodology (RSM) methods. A 2.5 fold (AU/mL) increase of bacteriocin was observed for sucrose (2.4 %) as carbon source and 4.7 fold (AU/mL) increased bacteriocin was observed in the presence of soyatone (1.03 %) as nitrogen source in the OFAT experiments. In order to increase bacteriocin production RSM tool was performed with optimized chemical and physical sources using Design expert 8.0.7.1. Soyatone (1.03 %), sucrose (2.4 %), pH (5.5) and temperature (34.5?ºC) condition yielded 25,600.34 AU/mL of bacteriocin from P. pentosaceus KC692718. This is the first report which has produced 20 fold increase of bacteriocin for Pediococcus pentosaceus KC692718 from that of MRS medium with 1?280 AU/mL.

SUBMITTER: Suganthi V 

PROVIDER: S-EPMC4444917 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature