Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway.

ABSTRACT:

Background

Wnt2 is overexpressed and able to promote tumorigenesis in many types of cancer. However, its expression and role in lung cancer has not been well clarified yet. In this study, we aims to investigate the expression pattern, clinical significance and the underlying molecular mechanism of Wnt2 in non-small lung cancer (NSCLC).

Methods

Immunohistochemical staining and ELISA assays were applied to detect Wnt2 level in tumor tissue and serum. EDU incorporation assays and colony formation assays were used to evaluate the growth-promoting effect of Wnt2 in vitro. Then we performed western blot and immunofluorescence assays to detect the activation of WNT signaling pathway. Finally mice engrafted with NSCLC tumor cells were used to assess the role of Wnt2 in vivo.

Resu

SUBMITTER: Huang C 

PROVIDER: S-EPMC4449433 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature