Human FMO2-based microbial whole-cell catalysts for drug metabolite synthesis.

ABSTRACT:

Background

Getting access to authentic human drug metabolites is an important issue during the drug discovery and development process. Employing recombinant microorganisms as whole-cell biocatalysts constitutes an elegant alternative to organic synthesis to produce these compounds. The present work aimed for the generation of an efficient whole-cell catalyst based on the flavin monooxygenase isoform 2 (FMO2), which is part of the human phase I metabolism.

Results

We show for the first time the functional expression of human FMO2 in E. coli. Truncations of the C-terminal membrane anchor region did not result in soluble FMO2 protein, but had a significant effect on levels of recombinant protein. The FMO2 biocatalysts were employed for substrate screening purposes, revealing tr

SUBMITTER: Geier M 

PROVIDER: S-EPMC4464233 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature