Drosophila Imp iCLIP identifies an RNA assemblage coordinating F-actin formation.

ABSTRACT:

Background

Post-transcriptional RNA regulons ensure coordinated expression of monocistronic mRNAs encoding functionally related proteins. In this study, we employ a combination of RIP-seq and short- and long-wave individual-nucleotide resolution crosslinking and immunoprecipitation (iCLIP) technologies in Drosophila cells to identify transcripts associated with cytoplasmic ribonucleoproteins (RNPs) containing the RNA-binding protein Imp.

Results

We find extensive binding of Imp to 3' UTRs of transcripts that are involved in F-actin formation. A common denominator of the RNA-protein interface is the presence of multiple motifs with a central UA-rich element flanked by CA-rich elements. Experiments in single cells and intact flies reveal compromised actin cytoskeletal dynamics

SUBMITTER: Hansen HT 

PROVIDER: S-EPMC4477473 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature