Hydrogen Exchange Mass Spectrometry of Proteins at Langmuir Monolayers.

ABSTRACT: Hydrogen exchange (HX) mass spectrometry (MS) is valuable for providing conformational information for proteins/peptides that are very difficult to analyze with other methods such as peripheral membrane proteins and peptides that interact with membranes. We developed a new type of HX MS measurement that integrates Langmuir monolayers. A lipid monolayer was generated, a peptide or protein associated with it, and then the monolayer-associated peptide or protein was exposed to deuterium. The deuterated species was recovered from the monolayer, digested, and deuterium incorporation monitored by MS. Test peptides showed that deuterium recovery in an optimized protocol was equivalent to deuterium recovery in conventional solution HX MS. The reproducibility of the measurements was high, despite t

SUBMITTER: Pirrone GF 

PROVIDER: S-EPMC4509961 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature