Developing a high-throughput screening method for threonine overproduction based on an artificial promoter.

ABSTRACT:

Background

L-Threonine is an important amino acid for animal feed. Though the industrial fermentation technology of threonine achieved a very high level, there is still significant room to further improve the industrial strains. The biosensor-based high-throughput screening (HTS) technology has demonstrated its powerful applications. Unfortunately, for most of valuable fine chemicals such as threonine, a HTS system has not been established mainly due to the absence of a suitable biosensor. In this study, we developed a HTS method to gain high-yielding threonine-producing strains.

Results

Novel threonine sensing promoters including cysJp and cysHp were discovered by proteomic analyses of Escherichia coli in response to extracellular threonine challenges. The HTS method was co

SUBMITTER: Liu Y 

PROVIDER: S-EPMC4546291 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature