Ontology highlight
ABSTRACT:
SUBMITTER: Putnam AA
PROVIDER: S-EPMC4546514 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Molecular cell 20150723 4
Most aspects of RNA metabolism involve DEAD-box RNA helicases, enzymes that bind and remodel RNA and RNA-protein complexes in an ATP-dependent manner. Here we show that the DEAD-box helicase Ded1p oligomerizes in the cell and in vitro, and unwinds RNA as a trimer. Two protomers bind the single-stranded region of RNA substrates and load a third protomer to the duplex, which then separates the strands. ATP utilization differs between the strand-separating protomer and those bound to the single-str ...[more]