Imaging Granzyme B Activity Assesses Immune-Mediated Myocarditis.

ABSTRACT:

Rationale

The development of molecular imaging approaches that assess specific immunopathologic mechanisms can advance the study of myocarditis.

Objective

This study validates a novel molecular imaging tool that enables the in vivo visualization of granzyme B activity, a major effector of cytotoxic CD8+ T lymphocytes.

Methods and results

We synthesized and optimized a fluorogenic substrate capable of reporting on granzyme B activity and examined its specificity ex vivo in mice hearts with experimental cytotoxic CD8+ T lymphocyte-mediated myocarditis using fluorescence reflectance imaging, validated by histological examination. In vivo experiments localized granzyme B activity in hearts with acute myocarditis monitored by fluorescent molecular tomography in conjunction

SUBMITTER: Konishi M 

PROVIDER: S-EPMC4553143 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature