Ontology highlight
ABSTRACT:
SUBMITTER: Magnuson B
PROVIDER: S-EPMC4675984 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Scientific reports 20151211
BruUV-seq utilizes UV light to introduce transcription-blocking DNA lesions randomly in the genome prior to bromouridine-labeling and deep sequencing of nascent RNA. By inhibiting transcription elongation, but not initiation, pre-treatment with UV light leads to a redistribution of transcription reads resulting in the enhancement of nascent RNA signal towards the 5'-end of genes promoting the identification of transcription start sites (TSSs). Furthermore, transcripts associated with arrested RN ...[more]