Chemokine (C-C Motif) Receptor 2 Mediates Dendritic Cell Recruitment to the Human Colon but Is Not Responsible for Differences Observed in Dendritic Cell Subsets, Phenotype, and Function Between the Proximal and Distal Colon.

ABSTRACT:

Background & aims

Most knowledge about gastrointestinal (GI)-tract dendritic cells (DC) relies on murine studies where CD103⁺ DC specialize in generating immune tolerance with the functionality of CD11b^+/- subsets being unclear. Information about human GI-DC is scarce, especially regarding regional specifications. Here, we characterized human DC properties throughout the human colon.

Methods

Paired proximal (right/ascending) and distal (left/descending) human colonic biopsies from 95 healthy subjects were taken; DC were assessed by flow cytometry and microbiota composition assessed by 16S rRNA gene sequencing.

Results

Colonic DC identified were myeloid (mDC, CD11c⁺CD123^-) and further divided based on CD103 and SIRPα (human a

SUBMITTER: Bernardo D 

PROVIDER: S-EPMC4705905 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature