Molecular Histochemistry Identifies Peptidomic Organization and Reorganization Along Striatal Projection Units.

ABSTRACT: Matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) (MALDI-IMS) provides a technical means for simultaneous analysis of precise anatomic localization and regulation of peptides. We explored the technical capability of matrix-assisted laser desorption ionization mass spectrometry for characterization of peptidomic regulation by an addictive substance along two distinct projection systems in the mouse striatum. The spatial expression patterns of substance P and proenkephalin, marker neuropeptides of two distinct striatal projection neurons, were negatively correlated at baseline. We detected 768 mass/charge (m/z) peaks whose expression levels were mostly negatively and positively correlated with expression levels of substance P and proenkephalin A (amino acids

SUBMITTER: Hishimoto A 

PROVIDER: S-EPMC4744103 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature