Design of a protocol for obtaining genomic DNA from saliva using mouthwash: Samples taken from patients with periodontal disease.

ABSTRACT:

Background

Obtaining high quality genomic DNA safely and economically is vital for diverse studies of large populations aimed at evaluating the role of genetic factors in susceptibility to disease.

Aim

This study was to test a protocol for the extraction of high quality genomic DNA from saliva samples obtained with mouthwash and taken from patients with periodontal disease.

Methods

Saliva samples were taken from 60 patients and then stored at room temperature. DNA extraction was carried out at distinct post-sampling times (10, 20 and 30 days). Evaluation of genomic DNA was performed with spectrophotometry, electrophoresis, and PCR genotyping and sequencing.

Results

The greatest concentration of DNA obtained was 352 μg at 10 days post-sampling, followed by 121.0

SUBMITTER: Mendoza AC 

PROVIDER: S-EPMC4862237 | biostudies-literature | 2016 May-Aug

REPOSITORIES: biostudies-literature