Concurrent SPECT/PET-CT imaging as a method for tracking adoptively transferred T-cells in vivo.

ABSTRACT:

Background

The ability of T-cells to traffic to and penetrate tumors impacts the clinical efficacy of T-cell therapy therefore methods to track transferred T-cells in vivo are needed. In this preliminary report, we evaluated the use of concurrent SPECT/PET-CT imaging to monitor the egress of HER-2/neu specific T-cells in a breast cancer patient with extensive bone-only metastatic disease.

Findings

Indium (In-111) labeled T-cells demonstrated similar or greater viability than unlabeled T-cells at either a low or high dose of In-111 over a 24-h incubation period in vitro. The function of labeled or unlabeled T-cells was not significantly different (p > 0.05) at either dose. T-cells trafficked to all sites of metastatic disease and infiltrated the tumor as assessed by SPECT ima

SUBMITTER: Stanton SE 

PROVIDER: S-EPMC4869363 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature