PET of Adoptively Transferred Chimeric Antigen Receptor T Cells with ⁸⁹Zr-Oxine.

ABSTRACT: Chimeric antigen receptor (CAR) T cell therapy is a promising clinical approach for reducing tumor progression and prolonging patient survival. However, improvements in both the safety and the potency of CAR T cell therapy demand quantitative imaging techniques to determine the distribution of cells after adoptive transfer. The purpose of this study was to optimize ⁸⁹Zr-oxine labeling of CAR T cells and evaluate PET as a platform for imaging adoptively transferred CAR T cells. Methods: CAR T cells were labeled with 0-1.4 MBq of ⁸⁹Zr-oxine per 10⁶ cells and assessed for radioactivity retention, viability, and functionality. In vivo trafficking of ⁸⁹Zr-oxine-labeled CAR T cells was evaluated in 2 murine xenograft tumor models: glioblastoma b

SUBMITTER: Weist MR 

PROVIDER: S-EPMC6167529 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature