MethylFlow: cell-specific methylation pattern reconstruction from high-throughput bisulfite-converted DNA sequencing.

ABSTRACT:

Motivation

DNA methylation aberrations are now known to, almost universally, accompany the initiation and progression of cancers. In particular, the colon cancer epigenome contains specific genomic regions that, along with differences in methylation levels with respect to normal colon tissue, also show increased epigenetic and gene expression heterogeneity at the population level, i.e. across tumor samples, in comparison with other regions in the genome. Tumors are highly heterogeneous at the clonal level as well, and the relationship between clonal and population heterogeneity is poorly understood.

Results

We present an approach that uses sequencing reads from high-throughput sequencing of bisulfite-converted DNA to reconstruct heterogeneous cell populations by assembling c

SUBMITTER: Dorri F 

PROVIDER: S-EPMC4892417 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature