Confirmation of translatability and functionality certifies the dual endothelin1/VEGFsp receptor (DEspR) protein.

ABSTRACT:

Background

In contrast to rat and mouse databases, the NCBI gene database lists the human dual-endothelin1/VEGFsp receptor (DEspR, formerly Dear) as a unitary transcribed pseudogene due to a stop [TGA]-codon at codon#14 in automated DNA and RNA sequences. However, re-analysis is needed given prior single gene studies detected a tryptophan [TGG]-codon#14 by manual Sanger sequencing, demonstrated DEspR translatability and functionality, and since the demonstration of actual non-translatability through expression studies, the standard-of-excellence for pseudogene designation, has not been performed. Re-analysis must meet UNIPROT criteria for demonstration of a protein's existence at the highest (protein) level, which a priori, would override DNA- or RNA-based deductions.

Methods

SUBMITTER: Herrera VL 

PROVIDER: S-EPMC4906906 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature