Sequencing of 15 622 gene-bearing BACs clarifies the gene-dense regions of the barley genome.

ABSTRACT: Barley (Hordeum vulgare L.) possesses a large and highly repetitive genome of 5.1 Gb that has hindered the development of a complete sequence. In 2012, the International Barley Sequencing Consortium released a resource integrating whole-genome shotgun sequences with a physical and genetic framework. However, because only 6278 bacterial artificial chromosome (BACs) in the physical map were sequenced, fine structure was limited. To gain access to the gene-containing portion of the barley genome at high resolution, we identified and sequenced 15 622 BACs representing the minimal tiling path of 72 052 physical-mapped gene-bearing BACs. This generated ~1.7 Gb of genomic sequence containing an estimated 2/3 of all Morex barley genes. Exploration of these sequenced BACs revealed that although dis

SUBMITTER: Munoz-Amatriain M 

PROVIDER: S-EPMC5014227 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature