Ex Vivo Maturation Assay for Testing Antimalarial Sensitivity of Rodent Malaria Parasites.

ABSTRACT: Ex vivo assay systems provide a powerful approach to studying human malaria parasite biology and to testing antimalarials. For rodent malaria parasites, short-term in vitro culture and ex vivo antimalarial susceptibility assays are relatively cumbersome, relying on in vivo passage for synchronization, since ring-stage parasites are an essential starting material. Here, we describe a new approach based on the enrichment of ring-stage Plasmodium berghei, P. yoelii, and P. vinckei vinckei using a single-step Percoll gradient. Importantly, we demonstrate that the enriched ring-stage parasites develop synchronously regardless of the parasite strain or species used. Using a flow cytometry assay with Hoechst and ethidium or MitoTracker dye, we show that parasite development is easily and rapidly

SUBMITTER: Chang ZW 

PROVIDER: S-EPMC5075096 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature