Why cannot a β-lactamase gene be detected using an efficient molecular diagnostic method?

ABSTRACT:

Objective

Fast detection of β-lactamase (bla) genes can minimize the spread of antibiotic resistance. Although several molecular diagnostic methods have been developed to detect limited bla gene types, these methods have significant limitations, such as their failure to detect almost all clinically available bla genes. We have evaluated a further refinement of our fast and accurate molecular method, developed to overcome these limitations, using clinical isolates.

Methods

We have recently developed the efficient large-scale bla detection method (_large-scaleblaFinder) that can detect bla gene types including almost all clinically available 1,352 bla genes with perfect specificity and sensitivity. Using this method, w

SUBMITTER: Park KS 

PROVIDER: S-EPMC5103155 | biostudies-literature | 2016 Sep-Oct

REPOSITORIES: biostudies-literature