Ontology highlight
ABSTRACT:
SUBMITTER: Zhu LW
PROVIDER: S-EPMC5109907 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Zhu Li-Wen LW Xia Shi-Tao ST Wei Li-Na LN Li Hong-Mei HM Yuan Zhan-Peng ZP Tang Ya-Jie YJ
Scientific reports 20161104
This study was initiated to improve E. coli succinate production by engineering the E. coli global transcription factor, Cra (catabolite repressor/activator). Random mutagenesis libraries were generated through error-prone PCR of cra. After re-screening and mutation site integration, the best mutant strain was Tang1541, which provided a final succinate concentration of 79.8 ± 3.1 g/L: i.e., 22.8% greater than that obtained using an empty vector control. The genes and enzymes involved in phosphoe ...[more]