Comparison of commercial nanoliquid chromatography columns for fast, targeted mass spectrometry-based proteomics.

ABSTRACT:

Aim

We compared four commonly used, commercially available reverse phase nanoLC columns for identification/determination of Wnt/β-catenin-related pathway proteins.

Materials & methods

The columns were: Chromolith^® (silica monolith; Merke Millipore, MA, USA), PepMap™ (porous particles; Thermo Fisher Scientific, MA, USA), Accucore™ (solid core particles; Thermo Fisher Scientific) and PepSwift™ (organic monolith; Thermo Fisher Scientific).

Results

The peak capacity of the columns varied from 100 (Pepswift) to 190 (Accucore) (for 30 min gradients). All columns enabled identification/detection of GSK3β and β-catenin in the complex samples. However, even the columns with higher peak capacities could not enable detection of the somewhat less abundant proteins AXIN

SUBMITTER: Vehus T 

PROVIDER: S-EPMC5137844 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature