A Benchtop Fractionation Procedure for Subcellular Analysis of the Plant Metabolome.

ABSTRACT: Although compartmentation is a key feature of eukaryotic cells, biological research is frequently limited by methods allowing for the comprehensive subcellular resolution of the metabolome. It has been widely accepted that such a resolution would be necessary in order to approximate cellular biochemistry and metabolic regulation, yet technical challenges still limit both the reproducible subcellular fractionation and the sample throughput being necessary for a statistically robust analysis. Here, we present a method and a detailed protocol which is based on the non-aqueous fractionation technique enabling the assignment of metabolites to their subcellular localization. The presented benchtop method aims at unraveling subcellular metabolome dynamics in a precise and statistically robust man

SUBMITTER: Furtauer L 

PROVIDER: S-EPMC5177628 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature