Myeloid C/EBPβ deficiency reshapes microglial gene expression and is protective in experimental autoimmune encephalomyelitis.

ABSTRACT:

Background

CCAAT/enhancer binding protein β (C/EBPβ) is a transcription factor that regulates the expression of important pro-inflammatory genes in microglia. Mice deficient for C/EBPβ show protection against excitotoxic and ischemic CNS damage, but the involvement in this neuroprotective effect of the various C/EBPβ-expressing cell types is not solved. Since C/EBPβ-deficient microglia show attenuated neurotoxicity in culture, we hypothesized that specific C/EBPβ deficiency in microglia could be neuroprotective in vivo. In this study, we have tested this hypothesis by generating mice with myeloid C/EBPβ deficiency.

Methods

Mice with myeloid C/EBPβ deficiency were generated by crossing LysMCre and C/EBPβ^fl/fl mice. Primary microglial cultures from C/EBPβ^fl/fl<

SUBMITTER: Pulido-Salgado M 

PROVIDER: S-EPMC5356255 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature