Miltefosine: a novel internal standard approach to lysophospholipid quantitation using LC-MS/MS.

ABSTRACT: Understanding and determining levels of lysophospholipids (LPLs) is of increasing interest to the bioanalytical community as they may be targeted for preparative removal as a matrix interference or as a lead substance as a biomarker of disease. Studies monitoring levels of LPLs have used a range of approaches for quantitation whereby those using an internal standard have used either deuterated analogues of the target LPL or alternative LPLs containing an odd number of carbon atoms within its chain, which can be expensive and difficult to distinguish with other LPLs, respectively. A structural analogue, miltefosine, was investigated as a novel internal standard to quantify a selection of lysophosphatidylcholines (LPCs) of clinical interest. A reverse phase C18 LC-MS/MS method was characteri

SUBMITTER: Godfrey AR 

PROVIDER: S-EPMC5366175 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature