Adoptive Transfer of mRNA-Transfected T Cells Redirected against Diabetogenic CD8 T Cells Can Prevent Diabetes.

ABSTRACT: Chimeric major histocompatibility complex (MHC) molecules supplemented with T cell receptor (TCR) signaling motifs function as activation receptors and can redirect gene-modified T cells against pathogenic CD8 T cells. We have shown that β₂ microglobulin (β₂m) operates as a universal signaling component of MHC-I molecules when fused with the CD3-ζ chain. Linking the H-2K^d-binding insulin B chain peptide insulin B chain, amino acids 15-23 (InsB_15-23) to the N terminus of β₂m/CD3-ζ, redirected polyclonal CD8 T cells against pathogenic CD8 T cells in a peptide-specific manner in the non-obese diabetic (NOD) mouse. Here, we describe mRNA electroporation for delivering peptide/β₂m/CD3-ζ genes to a reporter T cell line and purifie

SUBMITTER: Fishman S 

PROVIDER: S-EPMC5368593 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature