New var reconstruction algorithm exposes high var sequence diversity in a single geographic location in Mali.

ABSTRACT:

Background

Encoded by the var gene family, highly variable Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP1) proteins mediate tissue-specific cytoadherence of infected erythrocytes, resulting in immune evasion and severe malaria disease. Sequencing and assembling the 40-60 var gene complement for individual infections has been notoriously difficult, impeding molecular epidemiological studies and the assessment of particular var elements as subunit vaccine candidates.

Methods

We developed and validated a novel algorithm, Exon-Targeted Hybrid Assembly (ETHA), to perform targeted assembly of var gene sequences, based on a combination of Pacific Biosciences and Illumina data.

Results

Using ETHA, we characterized the repertoire of var genes in 12 samples from u

SUBMITTER: Dara A 

PROVIDER: S-EPMC5368897 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature