Polysulfides (H₂S_n) produced from the interaction of hydrogen sulfide (H₂S) and nitric oxide (NO) activate TRPA1 channels.

ABSTRACT: Hydrogen sulfide (H₂S) exerts synergistic effects with another gaseous signaling molecule nitric oxide (NO) on ion channels and vasculature. However, the mechanism of the synergy is not well understood. Here, we show that the interaction between H₂S and NO generates polysulfides (H₂S_n), which activate transient receptor potential ankyrin 1 (TRPA1) channels. High performance liquid chromatography with tandem mass spectrometry analysis, along with the imaging of intracellular Ca²⁺ and H₂S_n, showed that H₂S_n and their effects were abolished by cyanolysis and by reducing substances such as dithiothreitol (DTT), cysteine, and glutathione (GSH). However, the effects of nitroxyl or nitrosopersulfide

SUBMITTER: Miyamoto R 

PROVIDER: S-EPMC5380989 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature