On/off-switchable LSPR nano-immunoassay for troponin-T.

ABSTRACT: Regeneration of immunosensors is a longstanding challenge. We have developed a re-usable troponin-T (TnT) immunoassay based on localised surface plasmon resonance (LSPR) at gold nanorods (GNR). Thermosensitive poly(N-isopropylacrylamide) (PNIPAAM) was functionalised with anti-TnT to control the affinity interaction with TnT. The LSPR was extremely sensitive to the dielectric constant of the surrounding medium as modulated by antigen binding after 20?min incubation at 37?°C. Computational modelling incorporating molecular docking, molecular dynamics and free energy calculations was used to elucidate the interactions between the various subsystems namely, IgG-antibody (c.f., anti-TnT), PNIPAAM and/or TnT. This study demonstrates a remarkable temperature dependent immuno-interaction due to changes in the PNIPAAM secondary structures, i.e., globular and coil, at above or below the lower critical solution temperature (LCST). A series of concentrations of TnT were measured by correlating the ?LSPR shift with relative changes in extinction intensity at the distinct plasmonic maximum (i.e., 832?nm). The magnitude of the red shift in ?LSPR was nearly linear with increasing concentration of TnT, over the range 7.6?×?10-15 to 9.1?×?10-4?g/mL. The LSPR based nano-immunoassay could be simply regenerated by switching the polymer conformation and creating a gradient of microenvironments between the two states with a modest change in temperature.

SUBMITTER: Ashaduzzaman M 

PROVIDER: S-EPMC5382532 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature