Effect of Sodium Arsenite on the Expression of Antioxidant Genes (SOD2 and CAT) in MCF-7 and Jurkat Cell Lines.

ABSTRACT:

Background

Sodium arsenite (NaAsO2) has potent cytotoxic activity in human cancer cells. Oxidative stress has been suggested as a mechanism for arsenic-induced carcinogenesis. The purpose of the present study was to evaluate the alteration of mRNA levels of catalase (CAT) and superoxide dismutase 2 (SOD2) in MCF-7 and Jurkat cells after exposure to NaAsO₂.

Methods

Methylthiazol tetrazolium (MTT) viability assay was performed to evaluate cytotoxicity of NaAsO₂ in MCF-7 and Jurkat cells. For evaluating the expression levels of the CAT and SOD2, we used two concentrations of NaAsO₂ (5 and 15 μM), lower than the concentrations at which 50% of cell viability were lost. The cells were treated with co-treatment of NaAsO

SUBMITTER: Fallahzadeh-Abarghooei L 

PROVIDER: S-EPMC5402782 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature