Enterobacter cloacae Complex Isolates Harboring bla_NMC-A or bla_IMI-Type Class A Carbapenemase Genes on Novel Chromosomal Integrative Elements and Plasmids.

ABSTRACT: Carbapenem-resistant Enterobacter cloacae complex isolates submitted to a reference laboratory from 2010 to 2015 were screened by PCR for seven common carbapenemase gene groups, namely, KPC, NDM, OXA-48, VIM, IMP, GES, and NMC-A/IMI. Nineteen of the submitted isolates (1.7%) were found to harbor Ambler class A bla_NMC-A or bla_IMI-type carbapenemases. All 19 isolates were resistant to at least one carbapenem but susceptible to aminoglycosides, trimethoprim-sulfamethoxazole, tigecycline, and ciprofloxacin. Most isolates (17/19) gave positive results with the Carba-NP test for phenotypic carbapenemase detection. Isolates were genetically diverse by pulsed-field gel electrophoresis macrorestriction analysis, multilocus sequence typing, and hsp60

SUBMITTER: Boyd DA 

PROVIDER: S-EPMC5404547 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature