Ontology highlight
ABSTRACT:
SUBMITTER: Shen JP
PROVIDER: S-EPMC5449203 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Nature methods 20170320 6
We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics. We targeted all pairs of 73 cancer genes with dual guide RNAs in three cell lines, comprising 141,912 tests of interaction. Numerous therapeutically relevant interactions were identified, and these patterns replicated with combinatorial drugs at 75% precision. From these results, we anticipate that cellular context will be critical to syntheti ...[more]