The developmental regulator PKL is required to maintain correct DNA methylation patterns at RNA-directed DNA methylation loci.

ABSTRACT:

Background

The chromodomain helicase DNA-binding family of ATP-dependent chromatin remodeling factors play essential roles during eukaryote growth and development. They are recruited by specific transcription factors and regulate the expression of developmentally important genes. Here, we describe an unexpected role in non-coding RNA-directed DNA methylation in Arabidopsis thaliana.

Results

Through forward genetic screens we identified PKL, a gene required for developmental regulation in plants, as a factor promoting transcriptional silencing at the transgenic RD29A promoter. Mutation of PKL results in DNA methylation changes at more than half of the loci that are targeted by RNA-directed DNA methylation (RdDM). A small number of transposable elements and genes had reduced D

SUBMITTER: Yang R 

PROVIDER: S-EPMC5452414 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature