Simple, rapid and efficient transformation of genotype Nisqually-1: a basic tool for the first sequenced model tree.

ABSTRACT: Genotype Nisqually-1 is the first model woody plant with an available well-annotated genome. Nevertheless, a simple and rapid transformation of Nisqually-1 remains to be established. Here, we developed a novel shoot regeneration method for Nisqually-1 using leaf petiole and stem segment explants. Numerous shoots formed in the incision of explants within two weeks. The optimized shoot regeneration medium (SRM) contained 0.03 mg l^-1 6-benzylaminopurine, 0.02 mg l^-1 indole-3-butyric acid and 0.0008 mg l^-1 thidiazuron. Based on this, Agrobacterium-mediated genetic transformation of stem explants was examined using the vector pBI₁₂₁ that contains the β-glucuronidase (GUS) as a reporter gene. Consequently, factors affecting transformation frequency of

SUBMITTER: Li S 

PROVIDER: S-EPMC5453977 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature