Human memory CD8 T cell effector potential is epigenetically preserved during in vivo homeostasis.

ABSTRACT: Antigen-independent homeostasis of memory CD8 T cells is vital for sustaining long-lived T cell-mediated immunity. In this study, we report that maintenance of human memory CD8 T cell effector potential during in vitro and in vivo homeostatic proliferation is coupled to preservation of acquired DNA methylation programs. Whole-genome bisulfite sequencing of primary human naive, short-lived effector memory (T_EM), and longer-lived central memory (T_CM) and stem cell memory (T_SCM) CD8 T cells identified effector molecules with demethylated promoters and poised for expression. Effector-loci demethylation was heritably preserved during IL-7- and IL-15-mediated in vitro cell proliferation. Conversely, cytokine-driven proliferation of T_CM and T_SCM

SUBMITTER: Abdelsamed HA 

PROVIDER: S-EPMC5461005 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature