Respiratory Pathways Reconstructed by Multi-Omics Analysis in Melioribacter roseus, Residing in a Deep Thermal Aquifer of the West-Siberian Megabasin.

ABSTRACT: Melioribacter roseus, a representative of recently proposed Ignavibacteriae phylum, is a metabolically versatile thermophilic bacterium, inhabiting subsurface biosphere of the West-Siberian megabasin and capable of growing on various substrates and electron acceptors. Genomic analysis followed by inhibitor studies and membrane potential measurements of aerobically grown M. roseus cells revealed the activity of aerobic respiratory electron transfer chain comprised of respiratory complexes I and IV, and an alternative complex III. Phylogeny reconstruction revealed that oxygen reductases belonged to atypical cc(o/b)o₃ -type and canonical cbb₃ -type cytochrome oxidases. Also, two molybdoenzymes of M. roseus were affiliated either with Tt

SUBMITTER: Gavrilov S 

PROVIDER: S-EPMC5492636 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature