MicroRNA-33 Regulates Macrophage Autophagy in Atherosclerosis.

ABSTRACT:

Objective

Defective autophagy in macrophages leads to pathological processes that contribute to atherosclerosis, including impaired cholesterol metabolism and defective efferocytosis. Autophagy promotes the degradation of cytoplasmic components in lysosomes and plays a key role in the catabolism of stored lipids to maintain cellular homeostasis. microRNA-33 (miR-33) is a post-transcriptional regulator of genes involved in cholesterol homeostasis, yet the complete mechanisms by which miR-33 controls lipid metabolism are unknown. We investigated whether miR-33 targeting of autophagy contributes to its regulation of cholesterol homeostasis and atherogenesis.

Approach and results

Using coherent anti-Stokes Raman scattering microscopy, we show that miR-33 drives lipid droplet acc

SUBMITTER: Ouimet M 

PROVIDER: S-EPMC5494696 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature