RHD variants in Polish blood donors routinely typed as D-.

ABSTRACT:

Background

Blood donors exhibiting a weak D or DEL phenotypical expression may be mistyped D- by standard serology hence permitting incompatible transfusion to D- recipients. Molecular methods may overcome these technical limits. Our aim was to estimate the frequency of RHD alleles among the apparently D- Polish donor population and to characterize its molecular background.

Study design and methods

Plasma pools collected from 31,200 consecutive Polish donors typed as D- were tested by real-time polymerase chain reaction (PCR) for the presence of RHD-specific markers located in Intron 4 and Exons 7 and 10. RHD+ individuals were characterized by PCR or cDNA sequencing and serology.

Results

Plasma cross-pool strategy revealed 63 RHD+ donors harboring RHD*01N.03 (n = 17),

SUBMITTER: Orzinska A 

PROVIDER: S-EPMC5497842 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature