E2F1 somatic mutation within miRNA target site impairs gene regulation in colorectal cancer.

ABSTRACT:

Background

Genetic studies have largely concentrated on the impact of somatic mutations found in coding regions, and have neglected mutations outside of these. However, 3' untranslated regions (3' UTR) mutations can also disrupt or create miRNA target sites, and trigger oncogene activation or tumor suppressor inactivation.

Methods

We used next-generation sequencing to widely screen for genetic alterations within predicted miRNA target sites of oncogenes associated with colorectal cancer, and evaluated the functional impact of a new somatic mutation. Target sequencing of 47 genes was performed for 29 primary colorectal tumor samples. For 71 independent samples, Sanger methodology was used to screen for E2F1 mutations in miRNA predicted target sites, and the functional impact

SUBMITTER: Lopes-Ramos CM 

PROVIDER: S-EPMC5509303 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature